crispr offinder to run in linux online online with Winfy
We have hosted the application crispr offinder to run in linux online in order to run this application in our online workstations with Wine or directly.
Quick description about crispr offinder to run in linux online:
Summary: Design efficient and specific CRISPR small guide RNAs (sgRNAs) is one of the keys for a successful application of CRISPR technology. Importantly, more and more new RNA-guided endonucleases with different protospacer adjacent motif (PAM) have been discovered. Therefore, there is an urgent need to develop a versatile tool to design sgRNA to satisfy the requirement of different RNA-guided DNA endonucleases. To this end, a flexible sgRNA design program named “CRISPR-offinder” was developed. The most important feature of this new program is that it supports all known PAM types, as well as the customer-defined PAM. Besides, CRISPR-offinder can find and rank the candidate sgRNAs in genome by off-target sites number, and also can be used to design single or paired-gRNAs. CRISPR-offinder is freely available as a command-line program or accessible via our website.Availability: CRISPR-offinder is freely available at http: //crispr-offinder.com/ or http: //crispr.igenetech.com/.
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