crispr offinder

We have hosted the application crispr offinder in order to run this application in our online workstations with Wine or directly.

Quick description about crispr offinder:

Summary: Design efficient and specific CRISPR small guide RNAs (sgRNAs) is one of the keys for a successful application of CRISPR technology. Importantly, more and more new RNA-guided endonucleases with different protospacer adjacent motif (PAM) have been discovered. Therefore, there is an urgent need to develop a versatile tool to design sgRNA to satisfy the requirement of different RNA-guided DNA endonucleases. To this end, a flexible sgRNA design program named �CRISPR-offinder� was developed. The most important feature of this new program is that it supports all known PAM types, as well as the customer-defined PAM. Besides, CRISPR-offinder can find and rank the candidate sgRNAs in genome by off-target sites number, and also can be used to design single or paired-gRNAs. CRISPR-offinder is freely available as a command-line program or accessible via our website.
Availability: CRISPR-offinder is freely available at http: //crispr-offinder.com/ or http: //crispr.igenetech.com/.


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